Figure 8. The effects of Nsp14 inhibitors are potentiated when combined with remdesivir.

(A) Representative wells showing cells after no infection, or infection with remdesivir or vehicle control. DNA is visualised through staining by DRAQ7, and virus is visualised through staining for viral nucleoprotein (see Methods). It is known that <1 µM remdesivir is ineffective in Vero E6 Cells (25). (B) After seeding cells, media was washed and replaced with fresh media containing compounds at indicated concentrations, followed by infection with SARS-CoV-2. In addition, 0.5 µM remdesivir was added to all wells. Wells shown are representative of three biological repeats. DNA is visualised through staining by DRAQ7, and virus is visualised through staining for viral nucleoprotein (see Methods). (C) Quantification of viral and cell area through fluorescence microscopy of DNA staining by DRAQ7 and viral nucleoprotein (see Methods). DNA and viral area measurements were initially normalised to the vehicle control. Values were then plotted as a percentage of the maximum normalised measurement. IC₅₀ values are given for each compound in the presence of remdesivir. Points represent mean values; error bars give standard deviation over three biological repeats. Error bars are not given if the error is smaller than the size of the point.