Table 1. Thermodynamic parameters of the interaction of Δ21TgCEN1 and its N- and C-terminal domains with P17-XPC in the presence of 5 mM CaCl2 or 5 mM EGTA at 25°C.
| Buffer condition | n | Kd (nM) | ΔH (kJ mol−1) | −TΔS (kJ mol−1) | |
|---|---|---|---|---|---|
| Δ21TgCEN1 + P17-XPC | CaCl2 | n1 = 0.7 ± 0.1 | 1.3 ± 0.3 | −99.7 ± 5.3 | 48.6 ± 5.8 |
| n2 = 0.8 ± 0.1 | 4102 ± 914 | −50.8 ± 11.3 | 25.7 ± 11.5 | ||
| EGTA | 0.9 ± 0.1 | 59.1 ± 11.8 | −131.7 ± 1.6 | 89.9 ± 1.9 | |
| Δ21N-TgCEN1 + P17-XPC | CaCl2 | 0.7 ± 0.1 | 396.3 ± 60.9 | −74.25 ± 4.3 | 37.4 ± 3.9 |
| EGTA | - | - | - | - | |
| C-TgCEN1 + P17-XPC | CaCl2 | 0.8 ± 0.1 | 6.7 ± 1.1 | −95.7 ± 9.9 | 48.8 ± 10.2 |
| EGTA | 0.8 ± 0.1 | 50.1 ± 9.7 | −118.7 ± 2.9 | 76.5 ± 3.1 |
The reported parameters are the mean ± standard error of the mean (SEM) of at least three independent titrations using two different protein preparations.