Table 2. Kinetic parameters of PfA-M1, Pv-M1, Pb-M1, PfA-M17, Pv-M17 and Pb-M17 against unnatural amino acid substrate hCha.

	KM (µM)	kcat (s−1)	kcat/KM (M−1•s−1)
PfA-M1	96.3 ± 16.2	1.6 ± 0.2	1.7 × 104
Pv-M1	29.6 ± 4.4	22.4 ± 0.9	7.6 × 105
Pb-M1	53.9 ± 1.5	16.4 ± 2.9	3.0 × 105
PfA-M17	0.44 ± 0.02	0.21 ± 0.005	4.8 × 105
Pv-M17	1.1 ± 0.03	0.4 ± 0.04	3.6 × 105
Pb-M17	1.2 ± 0.1	0.3 ± 0.03	2.5 × 105

Assays carried out in biological triplicate and reported as mean ± standard deviation (SD).