Table 1. Examples of different proteins and expression systems successfully solubilised using polymers.

Expression organism	Membrane type	Target protein	Polymer	Reference
E. coli	Total membrane	BmrA	SMA2000, SMA3000, SZ25010, SZ30010, DIBMA	[18,19,26]
		ZipA	SMA2000, SMA3000, SZ25010, SZ30010, DIBMA	[16,18,19,26,28]
		KcsA	SMA2000	[13]
		AcrB	SMA2000	[21,29,30]
		bacteriorhodopsin	SZ25010	[31]
		SecYEG	SMA3000	[32]
		KimA	SZ30010	[33]
		YnaI	DIBMA	[34]
		GlpG	DIBMA, SZ25010, SZ30010, SMA2000	[35,36]
Rhodobacter sphaeroides	Total membrane	Cytochrome bc1	SZ30010	[37]
Flavobacterium johnsoniae	Total membrane	Alternative complex III	SMA3000	[38]
Thermosynechococcus elongatus	Thylakoid	Photosystem I	SMA1440	[39]
Sacchromyces cerevisiae	Mitochondrial	Cytochrome oxidase	SMA EF30	[40]
	Total membrane	Wsc1	SMA3000	[41]
Pichia pastoris	Total membrane	Adenosine 2A receptor	SMA2000	[42]
		Melatonin receptor	SMA2000	[43]
		CD81	SMA2000	[22]
Sf9 insect cells	Total membrane	hENT1/SLC29A1	SZ30010	[44]
		MRP4/ABCC4	SMA2000	[45]
		α1 glycine receptor	SZ30010	[46]
High five insect cells	Total membrane	P-glycoprotein/ABCB1	SMA2000	[47]
HEK293T cells	Total membrane	Dopamine receptor	SMA3000	[48]
		ABCG2	SMA2000	[49]
		Acid-sensing ion channel isoform 1	SZ30010	[50]
Hela cells	Whole cells	Total membrane solubilisaton	SZ30010	[51]
Red blood cells	Red blood cell ghosts	Rh complexes	SMA3000	[52]
Jurkat cells	Total membrane	Numerous cell surface proteins	SMA3000	[53]
Hamster brain	Tissue homogenate	Prion protein	SZ25010, SZ30010	[54]
Spinacia oleracea	Chloroplast thylakoid	Total protein	SMA1440, SZ25010, SZ30010, SMA2000	[27]
Sorghum bicolor	Total membrane	Dhurrin catalysing metabolon	SMA2000	[55]

SMA2000, SMA3000, SMA EF30 and SMA1440 polymers are from Cray Valley. SZ30010 and SZ25010 are from Polyscope. DIBMA is from BASF. SMA3000, SMA EF30 and SZ25010 have a styrene:maleic acid ratio of ∼3 : 1 whilst SMA2000 and SZ30010 have a ratio of 2 : 1 and 2.3 : 1, respectively. SMA1440 is a partially esterified variant of SMA [27]. DIBMA is a 1 : 1 alternating polymer of diisobutylene and maleic acid.