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Sample size of reference set. (A) Patients in discovery cohort were arranged by time, and a series of reference sets were established by extracting patients at 10-patient intervals. The reference set serves as the immune cell density distributions reference. Patients in discovery cohort and validation cohort used the reference set to get the modified Immunoscore; (B) C-index values change with the increase of reference set sample size. Ref set, reference set.
