Table 2.

Plasma and tissue biochemistry

	Cc1+/+	L-CC1	Cc1−/−	Cc1−/−xliver+
a) RD
Plasma TNFα (pg/ml)	4.6 ± 0.2	4.7 ± 0.2	9.0 ± 0.1 ab	4.5 ± 0.3 c
Plasma IL-6 (pg/ml)	34.6 ± 1.4	34.0 ± 0.7	57.1 ± 2.1 ab	33.8 ± 1.0 c
Plasma AST (mU/ml)	44.7 ± 5.2	36.7 ± 6.4	87.7 ± 5.0 ab	48.9 ± 7.5 c
Plasma ALT (mU/ml)	7.7 ± 1.2	8.2 ± 1.0	14.8 ± 0.5 ab	9.3 ± 1.5 c
Hepatic NO (uM/ugx 10−1)	4.0 ± 0.0	4.0 ± 0.0	2.0 ± 0.0 ab	3.0 ± 0.0 c
Hepatic GSH (umol/g wt)	1.8 ± 0.1	1.8 ± 0.0	1.8 ± 0.0	1.7 ± 0.0
Plasma ET-1 (pg/ml)	4.2 ± 0.4	3.6 ± 0.8	11.7 ± 0.4 ab	4.6 ± 0.5 c
b) HF
Plasma TNFα (pg/ml)	5.0 ± 0.2	5.5 ± 0.4	9.7 ± 0.3 ab	4.9 ± 0.2 c
Plasma IL-6 (pg/ml)	40.3 ± 1.3	39.8 ± 3.2	63.7 ± 1.7 ab	36.0 ± 2.5 c
Plasma AST (mU/ml)	77.6 ± 8.2	70.2 ± 9.6	135.7 ± 17.0 ab	58.9 ± 10.1 c
Plasma ALT (mU/ml)	16.8 ± 2.4	15.1 ± 1.6	27.0 ± 2.5 ab	13.4 ± 2.5 c
Hepatic NO (uM/ugx10−1)	5.0 ± 0.0	4.0 ± 0.0	2.0 ± 0.0 ab	5.0 ± 0.0 c
Hepatic GSH (umol/g wt)	4.3 ± 0.2	4.3 ± 0.2	1.6 ± 0.0 ab	4.3 ± 0.2 c
Plasma ET-1 (pg/ml)	15.6 ± 2.1	15.2 ± 2.4	33.7 ± 3.4 ab	13.6 ± 3.6 c

Male mice were fed a HF or kept on RD diet for 3 months starting at 3 months of age (n≥5–8 mice/genotype/feeding group). Retro-orbital blood and tissues were collected at 11:00 a.m. following an overnight fast. Values are expressed as mean±SEM.

^aP<0.05 vs Cc1^+/+

^bP<0.05 vs L-CC1

^cP<0.05 vs Cc1^−/−.