Figure 10.

Systemic exposure to NaIO₃ caused RPE cells to lose their normal structure and patterning. RPE cells chiefly died in the center to midperiphery in C57BL/6J mice. The RPE flatmounts were immunostained for ZO-1 (green), α-catenin (red), and Hoechst 33258 (blue). (A) RPE flatmounts from the PBS group. (B) High-resolution image from the PBS group, normally mononucleate and binucleate polygonal RPE cells did not exhibit a cytosolic α-catenin signal. (C) High-resolution image from the PBS group with only red channel (α-catenin). (D, F, H, J) Representative RPE flatmounts from NaIO₃-treated C57BL/6J mouse groups. (E, G, I, K) High-resolution images of the transition zone in RPE flatmounts from NaIO₃-treated groups. Scale bar in A, D, F, H, J: 1000 microns; scale bar in B, C, E, G, I, K: 200 microns.