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. 2021 Jul 5;8:697962. doi: 10.3389/fmolb.2021.697962

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Copyright © 2021 Chen and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CRISPR/dCas9-KRAB decreased the expression level of LINC00944 in RCC cells. (A,C) Expression levels of LINC00944 in RCC cell lines (OSRC, ACHN, Caki-1, Caki-2, 786-O, and 769-P) and normal renal tubular epithelial cell line (HK-2) by qRT-PCR and semi-quantitative RT-PCR. (B,D) Expression levels of LINC00944 in 786-O and 769-P RCC cells transfected with dCas9-KRAB and sgRNA-NT or sgRNA-LINC00944 for 24 h by qRT-PCR and semi-quantitative RT-PCR. *p < 0.05; **p < 0.01.