FIGURE 1.

Cannabis cigarette preparation and smoke exposure system. Cannabis cigarettes are prepared by a) grinding 0.84±0.06 g dried cannabis flower (10–14% tetrahydrocannabinol and 0% cannabidiol), b) packing into king-size empty cigarette tubes and twisting off the end to seal the cigarette. c) The filter of each cigarette is removed, and cannabis is packed towards the sealed end of the cigarette prior to smoke exposure. d) Cannabis cigarettes are loaded into the cigarette holder on a Smoke Inhalation Unit 24 system. Experimental animals are placed in the lower chamber. Cannabis smoke and fresh air are pumped into the chamber following a computerised protocol attached to the system.