FIGURE 6.

Impact of acute cannabis smoke exposure on immune mediators in the lungs. Male and female 6–8-week-old BALB/c mice were exposed to room air (RA) or the smoke of six cannabis cigarettes (CS) twice a day for 4 days. Immune mediators were quantified via multiplex analysis (Eve Technologies). Mediators shown are those with quantities above the lower level of detection of the analysis and are associated with a) smoke-associated inflammation, b) macrophage cell signalling, and c) antiviral signalling. d) Other immune mediators with significant differences above the lower level of detection are also shown. IL: interleukin; MCP: monocyte chemoattractant protein; MDC: macrophage-derived chemokine; MIP: macrophage inflammatory protein; IFN: interferon; IP-10: interferon-γ-inducible protein 10; TARC: thymus and activation-regulated chemokine; VEGF: vascular endothelial growth factor. Data represent mean±sem; n=5 per group. *p<0.05, unpaired t-test within each sex.