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. 2021 Jul 16;30:09636897211033778. doi: 10.1177/09636897211033778

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — SHR0302 regulated AKT and ERK1/2 pathways. The results of the phosphorylated protein array for human peripheral blood mononuclear cells (PBMCs) from 4 patients conditioned with vehicle or SHR0302 in vitro. After the original data is normalized by the software, fold change (FC) was used to screen the differential expressed proteins. GO enrichment analysis and KEGG pathway analysis were performed based on logFC and p value of these differential expressed proteins. (A) The horizontal and vertical coordinates represented the average expression (AveExp) levels of each group. Red represented the up-regulated protein, and blue indicated down-regulated protein, grey showed no significant difference. (B) The expression levels of phosphorylated proteins in the vehicle group and SHR0302 group: JAK1, Lck, Lyn, Pyk2, Syk, and Itk. (C) Gene Ontology (GO) can be divided into three parts: molecular function, biological process and cellular component. (D) The KEGG pathway with enrichment function obtained with phosphorylated protein array analysis. (E) Separated T cells and B cells were incubated with the different concentrations of SHR0302 (0, 5, 10 and 20 μM) for 48 h. Western blot analysis was performed to examine the protein levels of total-JAK1, phospho-JAK1, total-JAK2, phospho-JAK2, total-STAT1, phospho-STAT1, total-STAT3, phospho-STAT3, total-STAT5, phospho-STAT5, total-AKT, phospho-AKT, total-ERK1/2, phospho-ERK1/2 and GAPDH, respectively.