Figure 1.
Differential expression in murine splenic B cells of genes induced by LPS plus IL4 treatment and by YY1 knockout. RNA was isolated from naive yy1f/f splenic B cells, or cells stimulated with LPS plus IL4 for 3 days and prepared for RNA-seq analyses. Genes that were differentially regulated twofold or greater with a FDR of <0.05 are shown in the heatmap and in Supporting information Table 1. In addition, isolated splenic B cells from yy1f/f mice were either mock treated or treated with recombinant TAT-CRE protein to delete the yy1 gene. Cells were stimulated with LPS plus IL4 for 3 days and RNA was isolated for RNA-seq analyses. Differentially regulated genes are shown in the heatmap and in Supporting Information Table 2. Volcano plots show genes that increase (left) or decrease (right) after YY1 knockout. Red dots represent mitochondrial-related genes. RNA-seq was performed in triplicate from three independent experiments.