Figure 5.
YY1 knockout ex vivo minimally impacts mitochondrial respiration during the first three days. Splenic B cells from yy1f/f mice were either mock treated or treated with recombinant TAT-CRE protein to delete the yy1 gene. Cells were stimulated with LPS for 3 days then evaluated for (A) oxygen consumption rate, (B) extracellular acidification rate, (C) basal respiration, ATP coupled respiration, maximal respiration, or proton leak Seahorse Extracellular Flux (XF) Analyzer (Seahorse Bioscience, Billerica, MA, USA). Data are expressed as mean ± SEM of four values, and representative of three independent experiments with two mice per experiment. (D) Mitochondrial complex activities (complexes I and IV) were measured using isolated mitochondria from mock and TAT-CRE-treated B cells. Assays were performed in triplicate and repeated twice. Differences in each experiment did not reach statistical significance.