Table 1. Summary of TEM measurements of ID structures.
| Mean Standard Error (N) (Quartile range, Q1–Q3) | Baseline value for model fit (Range investigated) | |||||
|---|---|---|---|---|---|---|
| TEM measurement | Plicate | Interplicate | ID | |||
| Diameter (μm) | 9.83 ± 0.49 (53) (7.84–11.50) | 11.0 | ||||
| Region length (μm) | 1.76 ± 0.09 (207) (0.77–2.39) | 2.0 | 1.54 ± 0.08 (131) (0.90–2.01) | 1.0 | ||
| GJ length (μm)a | 0.21 ± 0.022 (48) (0.13–0.24) | 0.27 | 0.51 ± 0.034 (135) (0.26–0.63) | 0.52 | ||
| Intermembrane distance (nm)a | 17.25 ± 0.52 (154) (12.30–20.72) | 30b (15–60) | 14.16 ± 0.55 (91) (10.14–17.53) | 15 (7.5–60) | ||
| GJ membrane fraction (%)a | 11.05 ± 1.74 (16) (5.34–16.87) | 12 | 46.15 ± 2.55 (64) (29.82–62.61) | 45 | ||
| Fold frequency (folds/µm) | 2.51 ± 0.08 (92) (1.92–3.02) | 2.50 | ||||
| Fold amplitude (μm) | 0.17 ± 0.0070 (109) (0.12–0.22) | 0.18 | ||||
Data are pooled from images of 3 mouse hearts, 51 IDs (for intermembrane distance), and 76 IDs (for all other measurements).
a
Statistically significant differences (P < 0.01) between plicate and interplicate measurements.
b
Baseline model value for the plicate intermembrane separation parameter was based on our earlier work in atria (Veeraraghavan et al., 2018; Mezache et al., 2020).