Table 1. A comparison of feature sets of various demultiplexers.
| Software | Combinatorial
demultiplexing for both single- and paired-end data |
Unique 3’
barcodes for each 5’ barcode |
Remove
adaptors |
Quality
trim |
Move UMIs
to read header |
Multi-threaded |
|---|---|---|---|---|---|---|
| Cutadapt | No | No | Yes | Yes | Yes | Yes |
| Demultiplex | No | N/A | No | No | No | No |
| Flexbar 3.0 | No | No | Yes | Yes | Yes | Yes |
| FASTX-Toolkit | No | N/A | Yes | Yes | No | No |
| Btrim | No | Yes | Yes | Yes | No | No |
| Axe | No | Yes | No | No | No | No |
| deML | No | Yes | No | No | No | No |
| AdaptorRemoval2 | No | Yes | Yes | Yes | No | Yes |
| iCount demultiplex | Yes | Yes | Yes | No | Yes | No |
| Ultraplex | Yes | Yes | Yes | Yes | Yes | Yes |