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. 2021 Jul 5;12:701683. doi: 10.3389/fpls.2021.701683

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Copyright © 2021 Alvarado-Marchena, Marquez-Molins, Martinez-Perez, Aparicio and Pallás.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of RNA-protein complexes formed between purified GST:atALKBH9B_wt protein and the 3′UTR transcript of AMV-RNA3/4. (A) EMSA after incubation of 5 ng of 3′UTR transcript with no protein (lane 1) or with 5, 50, 100, 150, 200, 400, 560, 840, 980, and 1120 ng of GST:atALKBH9B_wt (lanes 2–11) corresponding to 0.01, 0.06, 0.12, 0.18, 0.24, 0.48, 0.67, 1.00, 1.17, and 1.22 μM, respectively. (B) Hill transformation. The thin line is the best fit determined by least-squares analysis, with the corresponding r coefficient and equation given in the insert. The slope of the best-fit equation determines a Hill coefficient of 1.35, indicating no or mild cooperativity, and the y-intercepted point gives the value of the K_d, which was 0.30 μM.