Figure 4.

CCR2 is required for efficient recruitment of activated Ly6C^lo monocytes to the lung during infection with O. tsutsugamushi. CCR2^-/- or C57BL/6 mice were infected as in Figure 3 . On CD11b⁺ Ly6C^lo cells from the lung, the expression of F4/80 (A) and MHCII (B) was measured by FACS. Shown are representative histograms from d 0, d 10 and d 17 p.i. (left panels), percentages of marker-positive Ly6C^lo cells (middle panels) and absolute numbers per lung wing (right panels). (C) Co-expression of F4/80 and MHCII on CD11b+ Ly6C^lo cells. Representative dotplots from d 0, d 10 and d 17 (left), percentages (upper right) and absolute numbers per lung wing (lower right panel). Data were pooled from 2 independent experiments (n=6 mice per genotype and time point, mean ± SD). *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001 by two way-ANOVA.