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. 2021 Jul 16;9(7):e002753. doi: 10.1136/jitc-2021-002753

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See https://creativecommons.org/licenses/by/4.0/.

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Transport profile of CHA-SME within Caco-2 cell monolayers. (A) CLSM images of Caco-2 cell monolayer after incubation with Cou-6-labeled CHA-SME. the cell nuclei and cytoskeleton were stained with DAPI (blue) and phalloidin (red), respectively. (B, C) The in vitro cellular uptake profile of Cou-6-labeled CHA-SME in lymphocytes determined by flow cytometry. Each value represents the mean±SEM (n=3). ***P<0.001 compared with the Cou-6. (D) The percentage of Cou-6-positive lymphocytes determined by flow cytometry. CHA-SME, chlorogenic acid-encapsulated SMEDDS; CLSM, confocal laser scanning microscopy.