Table 1.

Mean number of follicles after in vitro organotypic culture of fresh or cryopreserved whole ovaries

	Fresh			SF
	w/o cult	Cult 2 days	Cult 4 days	w/o cult	Cult 2 days	Cult 4 days
Primordial	28,94 ± 2,614	22,99 ± 1,853	14,62 ± 1,958a,c	22,4 ± 1,175	14,78 ± 1,587b,c	11,13 ± 1,29b
Primary	17,68 ± 2,068	15,26 ± 1,541	16,95 ± 2,471	18,04 ± 1,026	11,16 ± 1,219b	12,48 ± 1,155b
Secondary or more	53,37 ± 3,503	61,75 ± 2,48	68,44 ± 3,757a	59,56 ± 1,798	74,05 ± 2,346b,c	76,39 ± 2,163b

Fresh or SF (slow frozen/thawed) ovaries from FVB/N mice, 7-weeks old, n = 6–9 ovaries per group. w/o cult = without culture

Results are expressed in percentage of primordial, primary and secondary or more mature follicles relative to the total number of follicles present in one ovarian slide. Mean ± SEM

^adifferent from Fresh w/o cult, ^bdifferent from SF w/o cult, ^cdifferent from Fresh cult 2 days