Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The primers used for real-time PCR
| Gene name | Primer sequence (5’→3’) | Tm (℃) |
|---|---|---|
| β-actin |
F: GGCTGTATTCCCCTCCATCG R: CCAGTTGGTAACAATGCCATGT |
62 |
| EPO |
F: GGGTGTGAACTATATCGACACGTC R: TCAGAGTCAGAGTGTGCCTCG |
62 |
| IE180 |
F: CATCGTGCTGGACACCATCGAG R: ACGTAGACGTGGTAGTCCCCCA |
62 |
| LAT |
F: GGCAGCAGGACTACTGTCA R: GTCTTGGTGGGAAGAAGTA |
62 |
| TK |
F: ATGACGGTCGTCTTTGACCGCCAC R: CGCTGATGTCCCCGACGATGAA |
60 |
F forward primer, R reverse primer
