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. 2021 Jul 5;12:712030. doi: 10.3389/fmicb.2021.712030

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Copyright © 2021 Liu, Schubert, Könneke and Berg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SDS-PAGE (12.5%) of fractions obtained during purification of heterologously produced Nmar_1028. Lane 1, molecular-weight markers (labeled in kDa); lane 2, cells before induction; lane 3, cells after induction; lane 4, cell extract; lane 5, flowthrough from Ni-NTA column; lane 6, wash with 50 mM imidazole; lane 7, elution with 500 mM imidazole; lane 8, the first peak during gel filtration; lane 9, the second peak during gel filtration.