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Effect of pH on the forward (A) and reverse (B) reaction rates of recombinant (S)-3-hydroxybutyryl-CoA dehydrogenase Nmar_1028. (A) 100% activity corresponds to 69.6 ± 2.7 U mg– 1 protein measured with 0.2 mM (S)-3-hydroxybutyryl-CoA and 0.5 mM NAD+ at pH 7.8. (B) 100% activity corresponds to 54.9 ± 7.2 U mg– 1 protein measured with 0.2 mM acetoacetyl-CoA and 0.5 mM NADH at pH 7.8. Values are means ± standard deviations of results from three independent measurements.
