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. 2021 Jul 5;12:712030. doi: 10.3389/fmicb.2021.712030

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Copyright © 2021 Liu, Schubert, Könneke and Berg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic tree of (S)-3-hydroxybutyryl-CoA dehydrogenase Nmar_1028. The sequences are marked as following: purple triangle, Nmar_1028; red triangle, Msed_1423; red star, Msed_0399; olive star, Tneu_0541 (from Pyrobaculum neutrophilum); turquoise star, Igni_1058 (from Ignicoccus hospitalis); ◯, dehydrogenase; without ◯, fusion protein; 80 sequences from TACK group in red, 15 sequences from non-AOA Thaumarchaeota in fuchsia, 49 sequences from Euryarchaeota in blue, 1 sequence from bacteria in green. The scale bar represents a difference of 0.1 substitutions per site. The percentage bootstrap values for the clade of this group were calculated in 1000 replications. Only values above 70% are shown (as ⬤). The accession numbers are listed in Supplementary Table 1. The phylogenetic tree generated by maximum-parsimony algorithm (data not shown) was similar with minor exceptions.