. 2021 Jul 5;12:712030. doi: 10.3389/fmicb.2021.712030

TABLE 1.

Catalytic properties of heterologously produced (S)-3-hydroxybutyryl-CoA dehydrogenase Nmar_1028.

Substrate	V_max (U mg^–1 protein)	K_m (mM)	k_cat/K_m (s^–1 mM^–1)
Forward reaction ^a
(S)-3-Hydroxybutyryl-CoA (with 2 mM NAD)	98.6 ± 3.3	0.019 ± 0.002	3877
(S)-3-Hydroxybutyryl-CoA (with 0.5 mM NAD)	76.1 ± 3.4	0.017 ± 0.003	3344
NAD (with 0.2 mM (S)-3-hydroxybutyryl-CoA)	84.4 ± 2.8	0.10 ± 0.02	630
NADP (with 0.2 mM (S)-3-hydroxybutyryl-CoA)	10.1 ± 0.9	2.92 ± 0.63	3
Reverse reaction^b
Acetoacetyl-CoA (with 0.5 mM NADH)	144.8 ± 7.7	0.026 ± 0.004	4045
NADH (with 0.2 mM acetoacetyl-CoA)	112.9 ± 7.3	0.016 ± 0.006	5271

^aNo activity was detected with (R)-3-hydroxybutyryl-CoA (detection limit ≤ 0.012 U mg^–¹). The specific activity was 0.02 U mg^–¹ protein with 3-hydroxypropionyl-CoA. ^b≤ 0.006 U mg^–¹ protein with 5 mM NADPH.