. 2021 Jul 5;12:712030. doi: 10.3389/fmicb.2021.712030

TABLE 3.

Catalytic properties of various so-far-characterized 3-hydroxyacyl-CoA dehydrogenases toward 3-hydroxybutyryl-CoA and/or acetoacetyl-CoA.

Organism	Protein	Gene	3-Hydroxybutyryl-CoA			Acetoacetyl-CoA			References
			V_max/k_cat	K_m	k_cat/K_m	V_max/k_cat	K_m	k_cat/K_m
Nitrosopumilus maritimus SCM1	HBDH	Nmar_1028	98.6/74 (2 mM NAD) 76.1/57 (0.5 mM NAD)	0.019 (2 mM NAD)0.017 (0.5 mM NAD)	3,877 (2 mM NAD) 3,344 (0.5 mM NAD)	144.8/108	0.026	4,160	This work
Metallosphaera sedula DSM 5348	HBDH	Msed_1423	96/75(0.5 mM NAD)	0.005	14,957	NR	NR	NR	Liu et al., 2020
	CCH/HBDH	Msed_0399	76/90^a 22.6/19^a 27.6/33^a	0.12 0.060.2	748 445163	NR	NR	NR	Ramos-Vera et al., 2011; Hawkins et al., 2014; Liu et al., 2020
Ignicoccus hospitalis	CCH/HBDH	Igni_1058	117/152	0.086	1,800	NR	NR	NR	Flechsler et al., 2021
Cupriavidus necator H16	HBDH/enoyl-CoA hydratase^b	H16_A0461	NR	NR	NR	149/216	0.048	4,500	Volodina and Steinbüchel, 2014
	HBDH	RePaaH1	NR	NR	NR	85.4/46	0.018	2,530	Kim J. et al., 2014
Clostridium butyricum	HBDH	CbHBD	NR	NR	NR	56.8/29	0.028	1,035	Kim E. J. et al., 2014
Caenorhabditis elegans Bristol N2	HADH	cHAD	NR	NR	NR	78/45	0.072	600	Xu et al., 2014
Mycobacterium tuberculosis H37Rv	HBDH	FadB2	0.19/0.096	0.0435	2	0.13/0.065	0.0656	1	Taylor et al., 2010
Escherichia coli ATCC 11775	Fatty acid oxidation complex	?	NR	NR	NR	64/288	0.066	4,364	Binstock and Schulz, 1981
Mycolicibacterium smegmatis ATCC 14468	HADH	?	NR	NR	NR	12.5/10	0.036	291	Shimakata et al., 1979
Homo sapiens	Short-chain HADH I	SCHAD I	NR/119	0.0225	5,290	NR/1,287	0.0166	77,500	Filling et al., 2008
	Short-chain HADH II	SCHAD II	NR/4.83	0.0852	58	NR/23.8	0.0257	927	Filling et al., 2008
	Short chain L-HADH	rSCHADH6	NR	NR	NR	459/252	0.0187	13,500	Barycki et al., 1999
	Short chain L-HADH	SCHAD	NR/0.011	0.043	0.26	NR	NR	NR	He et al., 1999
	L-HADH	HBHAD	NR	NR	NR	NR/37	0.089	416	He et al., 1998
Rattus norvegicus	HADH	HAD	NR	NR	NR	976/569	0.044	13,000	Liu et al., 2004
	HADH I	?	270/144	0.0699	2,060	820/437	0.0169	25,878	Osumi and Hashimoto, 1980
	HADH II	?	15.6/20	0.0422	474	23.5/30	0.0397	760	Osumi and Hashimoto, 1980
Sus scrofa	L-HADH	HAD	NR	NR	NR	NR/330	0.003	110,000	He and Yang, 1998
	L-HADH	?	39/21	0.0072	2,889	230/123	0.0118	10,395	He et al., 1989
	L-HADH	?	NR	NR	NR	426/227	0.06	3,787	Noyes and Bradshaw, 1973

V_max, U mg^–¹ protein; k_cat, s^–¹; K_m, mM. (S)-3-hydroxybutyryl-CoA dehydrogenase, HBDH; crotonyl-CoA hydratase, CCH; 3-hydroxyacyl-CoA dehydrogenase, HADH?, unknown; NR, not reported. ^aThe V_max values are normalized to 75°C based on the assumption that a 10°C rise in temperature doubles the reaction rate. ^bSpecific activity with crotonyl-CoA was 98 U mg^–¹.