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. 2021 Jul 5;9:687169. doi: 10.3389/fcell.2021.687169

FIGURE 5.

FIGURE 5

Transactive response DNA-binding protein∼43 kDa (TDP43) strengthens lipid uptake of macrophages through promoting Cluster of Differentiation 36 Receptor (CD36) expression. (A) qPCR detection of lipid uptake and export related genes in macrophages transfected with si-TDP43 or si-nc for 24 h and later incubated with 40 μg/mL oxLDL for 24 h. Data are represented as means ± SD (n = 3; *P < 0.05 vs. si-nc + oxLDL group). (B) Western blot analysis of lipid uptake and export related proteins in macrophages transfected with si-TDP43 or si-nc for 24 h and later incubated with 40 μg/mL oxLDL for 24 h. Data are represented as means ± SD (n = 3; *P < 0.05 vs. si-nc + oxLDL group). (C) Western blot analysis of CD36, MSR1, and OLR1 protein expressions in macrophages infected with lenti-control or lenti-TDP43 for 48 h. Data are represented as means ± SD [n = 3; *P < 0.05 vs. lenti-TDP43 (–) group]. (D) Immunofluorescence analysis of Dil-oxLDL uptake of macrophages infected with lenti-control or lenti-TDP43 for 48 h. Data are represented as means ± SD [n = 50; *P < 0.05 vs. lenti-TDP43 (–) group]. Scale bar = 100 μm. (E) Immunofluorescence analysis of Dil-oxLDL uptake of macrophages incubated with or without CD36 specific inhibitor (sulfo-N-succinimidyl oleate, SSO, 50 μM) and later infected with lenti-control or lenti-TDP43 for 48 h. Data are represented as means ± SD (n = 50; *P < 0.05 vs. lenti-TDP43 group). Scale bar = 100 μm.