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. 2021 Jul 5;15:691931. doi: 10.3389/fnins.2021.691931

FIGURE 3.

FIGURE 3

Chemogenetic activation of PV interneurons alleviated anxiety-like behavior in rats with chronic inflammatory pain. (A) A representative figure showing the expression of mCherry in the ACC (whole figure scale bars: 500 μm; local figure scale bars: 20 μm). (B) Representative images of PV interneurons (green) merged with mCherry (red) in the ACC in the model rats (scale bars: 20 μm). (C) A schematic of the experimental design. (D) PWTs changes with chemogenetic activation of PV neurons in the ACC (n = 11 in the model-PV-mCherry-CNO group; n = 12 in the model-PV-hM3D(Gq)-CNO group). (E) The trajectories of rats in the OF. (F) The trajectories of rats in the EZM. (G) Quantification of behavioral parameters in the OF test (n = 8 in the model-PV-mCherry-CNO group; n = 8 in the model-PV-hM3D(Gq)-CNO group). (a) The time spent in the central area, (b) the percentage of distance traveled in the central area, and (c) the total distance traveled throughout the arena. (H) Quantification of behavioral parameters in the EZM test (n = 8 in the model-PV-mCherry-CNO group; n = 8 in the model-PV-hM3D(Gq)-CNO group). (a) The time spent in the open arm and (b) the percentage of distance traveled in the open arm (I) Quantification of behavioral parameters in the NSF test (n = 9 in the model-PV-mCherry-CNO group; n = 10 in the model-PV-hM3D(Gq)-CNO group). (a) The latency to initiate feeding and (b) food consumption. All data represent the mean ± SEM; ∗P < 0.05 compared to the model-PV-mCherry-CNO group.