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. Author manuscript; available in PMC: 2021 Jul 19.
Published in final edited form as: Cell Rep. 2021 Jun 29;35(13):109329. doi: 10.1016/j.celrep.2021.109329

Figure 5. Methylation silences DINO in human cancers.

Figure 5.

(A) Correlation coefficient for methylation at each CpG near the DINO DMR with DINO and CDKN1A expression in SARC samples, (DINO correlation: cg13662121 p = 3 × 10−6, cg05460965 p = 2 × 10−6, cg11920449 p = 3 × 10−5, cg24425727 p = 3 × 10−4, cg15474579 p = 1 × 10−6, cg18900812 p = 0.02; CDKN1A correlation: cg11706349 p = 0.01, cg23305046 p = 0.01). Pearson correlation two-tailed p value.

(B) Correlation of average DINO methylation with DINO expression in SARC (r = −0.32, p = 8.5 × 10−7). Pearson correlation two-tailed p value.

(C) Correlation of average DINO methylation with CDKN1A expression in SARC (r = 0.01, p = 0.94). Pearson correlation two-tailed p value.

(D) Methylation as measured by bisulfite sequencing at CpGs within the DINO DMR and TSH2B CpG island in HT-1080 cells. Cells were infected with lentivirus expressing either TET1-dCas9 or dead TET1-dCas9 (dTET1-dCas9) and a sgRNA targeting the DINO DMR. Above: map of the location of sgRNA within the DINO DMR. (mean ± SD, cg11920449, p = 0.004, cg24425727, p = 0.01, two-tailed t test).

(E) Expression of DINO and CDKN1A in HT-1080 cells stably expressing TET1-dCas9 or dTET1-dCas9 with or without a sgRNA targeting the DINO DMR. (mean ± SD, *p = 3.9 × 10−7, two-tailed t test).

(F) Expression of select p53-regulated genes located on different chromosomes than the DINO/CDKN1A locus. (mean ± SD, TNFRSF10C p = 6.4 × 10−5, GADD45A p = 8.7 × 10−8, DDB2 p = 0.0027, CCNG1 p = 0.0006, two-tailed t test).

See also Figure S5.