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. 2021 Jul 19;17(7):e9832. doi: 10.15252/msb.20209832

Figure EV5. The sigV feedback loop increases the dynamic range of the circuit.

Figure EV5

  • A
    Sketch of WT sigV circuit. Where R stands of RsiV.
  • B
    Sketch of rewired circuit with deleted positive feedback loop. Where R stands for RsiV and Pspank for the IPTG inducible promoter spank.
  • C
    The fold change in sigV expression pre/post 1 μg/ml lysozyme stress was calculated from model simulations of the WT system (blue line) and a feedback‐broken system where the levels of the sigV operon are inducible (red line). The shaded areas represent one standard deviation of mean. N = 100 simulation.
  • D
    The experimental fold change for the WT, and the feedback‐broken strain for IPTG values between 0 to 1,000 μM. The blue line is the WT fold change, and the red line is the fold change of ΔsigVrsiVoatAyrhK P spank ‐sigVrsiVoatAyrhK. The shaded areas represent one standard deviation of mean. n = 3 biological repeats for all data shown.

Data information: For more information on the number of repeats, please see Appendix Table S4.