TABLE 1.
Gram Staining | Gram-Positive Rods | ||
Catalase test | Negative | ||
Tolerance to acidic pH | Log CFU/ml–1 | ||
0 h | 1 h | 3 h | |
pH 2 | 6.95 ± 1.31 | 4.15 ± 0.51 | 3.22 ± 1.20 |
pH 3 | 7.86 ± 1.52 | 6.36 ± 1.33 | 4.94 ± 1.01 |
pH 6 | 6.21 ± 2.34 | 6.13 ± 0.52 | 5.71 ± 1.91 |
Bile salt tolerance | Log CFU/ml–1 | ||
0 h | 4 h | ||
2% | 6.89 ± 0.52 | 5.29 ± 2.11 | |
4% | 7.06 ± 1.14 | 6.17 ± 1.26 | |
Hemolytic test | Negative | ||
Carbohydrate fermentation | Source | Growth | |
Glucose | +++ | ||
Xylose | +++ | ||
Arabinose | ++ | ||
Mannose | ++ | ||
Galactose | +++ | ||
Cellobiose | ++ | ||
Xylitol | + | ||
Arabitol | + | ||
Lactose | ++ | ||
Cell surface hydrophobicity (%) | 65.90 ± 1.51 | ||
Auto aggregation (%) | 58.99 ± 1.34 | ||
Antimicrobial activity | Zone of inhibition (mm) | ||
Escherichia coli ATCC 25922 | Staphylococcus aureus ATCC 6538 | Salmonella Paratyphi ATCC 9150 | |
18 | 21 | 19 | |
Survival in GIT conditions | Survival (%) | ||
0 h | 3 h | 6 h | |
84.6 ± 0.61 | 68.4 ± 1.34 | 64.7 ± 2.01 |
+ weak growth; ++ good growth; +++ very good growth. Data shown are mean ± SE of triplicate values of independent experiments.