Table 1.
Comparison of dCas12a efficiency when expressed from plasmid (pC-NLS, pC-NLS-Mxi1) or integrated into the genome
| Fold repression | eGFP fluorescence (103 MEFL) | ||||
|---|---|---|---|---|---|
| gENO2 | gENO6 | gENO2 | gENO6 | gNone | |
| Integrated dCas12a-NLS | 8 ± 0.7 | 14.9 ± 1.1 | 21.6 ± 3.1 | 11.5 ± 1.8 | 171.7 ± 17.3 |
| Plasmid-based dCas12a-NLS | 2.2 ± 0.1 | 3.5 ± 0.2 | 37.7 ± 2.4 | 23.5 ± 3.3 | 81.8 ± 2.6 |
| Integrated dCas12a-NLS-Mxi1 | 12.6 ± 0.7 | 8.2 ± 0.5 | 13.2 ± 0.8 | 20.3 ± 1.3 | 166.5 ± 18.7 |
| Plasmid-based dCas12a-NLS-Mxi1 | 4.5 ± 0.3 | 2.3 ± 0.2 | 14.1 ± 1.1 | 27.3 ± 4.5 | 63.6 ± 8.1 |
Fold repression of eGFP expressed as an average of values normalized by a non-targeting gRNA (gNone) for four biological replicates ± 1 standard error.