Table 1.
pH dependence of synthetic TFOs. Tm values (ºC) calculated at different pH values for the triplexes containing either C+-GC or Z-GC triplets at isolated or contiguous positions. Values in parenthesis indicate TFO dissociation in melting profiles where the TFO dissociates before melting of the underlying duplex. Representative fluorescence melting profiles are shown in Figure 3
| pH 5.0 | pH 6.0 | pH 7.0 | pH 8.0 | pH 9.0 | ||
|---|---|---|---|---|---|---|
| Isolated | Duplex | 73.0 | 75.1 | 75.3 | 73.6 | 73.2 |
| C+-GC | 74.2 | 75.3 (54.9) | 75.2 | 74.2 | 74.4 | |
| Z-GC | 80.6 | 81.9 | 80.8 | 78.4 | 77.4 | |
| Contiguous | Duplex | 73.5 | 75.3 | 75.1 | 74.7 | 74.1 |
| C+-GC | 73.1 (63.5) | 75.4 (43.8) | 75.1 | 75.0 | 74.6 | |
| Z-GC | 78.1 | 79.2 | 78.5 | 77.0 | 76.5 | |