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pH dependence of enzymatically-generated TFOs. Tm values (ºC) calculated at different pH values for the triplexes containing either C+-GC or Z-GC triplets at isolated positions. Representative fluorescence melting profiles are shown in Figure 6A
| pH 5.0 | pH 7.5 | pH 9.0 | |
|---|---|---|---|
| Duplex | 50.6 | 52.7 | 51.4 |
| C+-GC | 53.4 | 51.1 | 51.1 |
| Z-GC | 66.1 | 64.7 | 64.0 |
