Figure 6.

Depletion of CCNC does not restore HR repair. (A) Depletion of CCNC does not affect RAD51 foci formation upon DNA damage. HEK293A BRCA2 mAID CCNC-KO and WT cells with BRCA2 depletion (+IAA) or without BRCA2 depletion were treated with Olaparib (2 μM) for 24 h and collected for immunostaining with RAD51 antibody. (B) Statistical quantification of percentage of cells with >10 RAD51 foci from (A). (C) Validation of CCNC and BRCA2 knock down efficiency in U2OS DR-GFP cells by western blot with indicated antibodies. (D) Statistical quantification of HR repair efficiency in cells from (C). Data are represented as mean ± SD (n = 3). ns, not significant (Student t-test).