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. 2020 Jul 3;27(3):388–406. doi: 10.1093/ibd/izaa161

FIGURE 7.

FIGURE 7.

The effects of APX3330 treatment on APE1/Ref-1 expression in myenteric neurons of Winnie mice. A, APE1/Ref-1 labeled by immunofluorescence (red) in the colon cross-sections. A’, APE1/Ref-1 immunofluorescence (green) in the myenteric plexus in wholemount LMMP preparations. B, Density of APE1/Ref-1 fluorescence in the colon cross-sections relative to the total area of colonic mucosa in C57BL/6, Winnie sham-treated, and Winnie APX3330-treated mice (n = 5/group; scale bar = 100 µm, 20x magnification). C, Density of APE1/Ref-1 fluorescence quantified as a percentage relative to the ganglion area in the myenteric plexus in C57BL/6 control, Winnie sham-treated, and Winnie APX3330-treated mice (n = 5/group; scale bar = 50 µm, 40x magnification). Data expressed as mean ± SEM, *P < 0.05, ****P < 0.0001 compared with C57BL/6 control mice; ^^^P < 0.001 compared with Winnie sham-treated mice.