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. 2021 Jul 6;49(13):7298–7317. doi: 10.1093/nar/gkab549

Figure 1.

Figure 1.

Sel-TCP-seq analysis of eIF3 subunits in HEK293T cells. (A) Schematic representation of the Sel-TCP-seq protocol for analysis of eIF3D-V5 and eIF3B. IP, immunoprecipitation. (B) Metagene plots for eIF3D-V5 and eIF3B footprints. All reads were mapped to all human protein-coding transcripts with a 5′-UTR and 3′-UTR of >100 nt (n = 37 916). (C) Correlation of scanning efficiency (SE) for eIF3D-V5 with that for eIF3B. All human protein-coding transcripts with a coefficient of variation of >0.5 in two technical replicates were plotted (n = 34 574). Pearson's correlation coefficient (r) is indicated. (D) Percentage of Met-tRNAi reads for tRNAs bound to eIF3D-V5 or eIF3B complexes. NS, not significant (Student's t test). (E) Percentage of reads mapped to CDS for eIF3D-V5 and eIF3B footprints. The P value was determined with Student's t test.