Skip to main content
. 2021 Jul 6;49(13):7298–7317. doi: 10.1093/nar/gkab549

Figure 7.

Figure 7.

Contribution of eIF2A and eIF2D to translation initiation at near-cognate codons in the setting of eIF2 inhibition. (A) Numbers of ORFs showing a significant change (adjusted P value of <0.05) in translation efficiency in eIF2A KO, eIF2D KO and DKO cells compared with control cells under the arsenite-treated condition. ORFs not commonly up-regulated or down-regulated in two clones were removed. (B) Read aggregation plots for GTI-seq and Sel-TCP-seq of eIF3D-V5 for RPL38 mRNA. Red and blue circles show GTI-seq peaks and 40S decreasing points, respectively; black inverted triangles indicate TIS positions identified by TISCA; and red and black vertical lines denote novel TIS positions identified by TISCA and canonical TIS positions, respectively. euORF, extended upstream ORF. (C) Read aggregation plots of Ribo-seq for RPL38 mRNA in WT and DKO cells treated with arsenite. The average of two replicates is shown. (D) Fold change in translation efficiency for ORFs of RPL38 as analyzed by RiboDiff in eIF2A KO, eIF2D KO, and DKO cells with or without arsenite treatment compared with control cells not treated with arsenite.