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. 2021 Apr 20;49(13):e74. doi: 10.1093/nar/gkab262
Cell lines, plasmids and si-RNA Source Catalog number
HAP1 WT Horizon Discovery Cat #: C631
HAP1 POLQKO Horizon Discovery Cat #: HZGHC000647c005
HAP1 LIG4KO Horizon Discovery Cat #: HZGHC000759c005
HAP1 RAD52KO Horizon Discovery Cat #: HZGHC000623c002
DLD-1 WT Horizon Discovery Cat #: CCL-221
DLD-1 BRCA2 KO Horizon Discovery Cat #: HD 105–007
U2OS WT Gift from Jeremy Stark (20) NA
U2OS POLQKO Gift from Jeremy Stark (20) NA
U2OS RAD52KO Gift from Jeremy Stark (20) NA
U2OS EJ-2 Gift from Jeremy Stark (8) NA
HCC1937 pcDNA3.1 Previous publication (21) NA
HCC1937 BRCA1 complemented Previous publication (21) NA
HEK293T ATCC Cat #: CRL-3216
AAVS1 T2 CRISPR in pX330 Addgene Cat #: 72833
pUC57 DS-121 Made for this paper (Genscript) N/A
ON-TARGETplus Human BRCA1 (672) siRNA - SMARTpool Dharmacon Cat #: L-003461
ON-TARGETplus Non-targeting siRNA #1 Dharmacon Cat #: D-001810