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. 2021 Jul 1;49(13):7791–7805. doi: 10.1093/nar/gkab535

Figure 4.

Figure 4.

Monitoring the repair process of multi-invasion-induced integration of multiple DNA fragments. (A) Scheme of genome assembly a KanMX cassette from three fragments for monitoring the repair process of multi-invasion-induced rearrangement process. (B) The CFU number for integration of single fragments in strains with MPH1 deletion and/or PpRAD52 overexpression. (C) The CFU number for integration of three fragments in strains with MPH1 deletion and/or PpRAD52 overexpression. The transformed cells were incubated in recovery medium for 1, 1.5 and 2 h to monitor the repair process.

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