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. 2021 Jul 1;49(13):7791–7805. doi: 10.1093/nar/gkab535

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — Engineering fatty alcohol biosynthesis in P. pastoris with characterized neutral sites and promoters. (A) The corresponding eGFP signal integral with different neutral sites and promoters. (B) Two-factorial regulation of fatty alcohol biosynthesis by tuning the expression of FaCoAR with different neutral sites and promoters. The fatty alcohols were extracted from 72 h cultivated cells at 30°C, 220 rpm. The cells were cultivated in Delft minimal medium with 20 g/l glucose. All data represent the mean±s.d. of three yeast clones.