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. 2021 Apr 27;49(13):e75. doi: 10.1093/nar/gkab274

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Detection sensitivity of A-Star for rare SNV enrichment. (A) Evaluation of enrichment efficiency of A-Star with KRAS G12D samples of varying VAFs by TaqMan qRT-PCR. (B) The values of fold increases were calculated from the data in (A). (C) Evaluation of enrichment efficiency of A-Star with KRAS G12D samples of varying VAFs by Sanger sequencing. (D, E) Comparison enrichment efficiency of A-Star with the uncoupled PfAgo-PCR tested with 1% VAF samples of KRAS G12D by TaqMan qRT-PCR (D) and Sanger sequencing (E). (F) Correlation of the threshold cycle (Ct) value and KRAS G12D samples of varying VAFs by TaqMan qRT-PCR. The no-template control (NTC) contained only water. Error bars represent the mean ± S.D., n = 3.