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. 2021 Apr 27;49(13):e75. doi: 10.1093/nar/gkab274

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Analysis of A-Star with human clinical samples. (A) Flow chart of the A-Star procedure for the identification of cancer-associated SNVs in human samples. (B) Evaluation of A-Star by TaqMan qRT-PCR using clinical samples from diverse cancer types, as exemplified by KRAS G12D-containing samples from patients with lung adenocarcinoma (LUAD), colorectal cancer (CRC), or endometrial cancer (EMC). The control reactions did not contain gDNA pairs. Error bars represent standard deviations of the mean, n = 3.