(a) Indirect calorimetric measurement on wild-type vs Adipo-Alpl KO mice that had been on HFD for 4 weeks at 22 °C; n=7 mice for wild-type and 6 mice for Adipo-Alpl KO; the grey area indicates the dark period.
(b) Averaged respiration rates over 24 hours as measured in (a); n=7 mice for wild-type and 6 mice for Adipo-Alpl KO.
(c) Cumulative movement of mice over 24 hours; n=7 mice for wild-type and 6 mice for Adipo-Alpl KO.
Data are presented as means ± SEM. Statistical significance was calculated by either two-way ANOVA (a) or unpaired Student’s two-sided t-test (b and c).