Figure 2.

Treatment of zebrafish larvae with LPS causes increased RGC branching dynamics. A, Experimental design: single RGCs expressing mYFP were imaged at 3dpf before and after 2 h treatment with LPS or mock treatment control. B–E, Arbors are shown as projected z stacks (B,D) and as reconstructed skeletons (C,E). Green represents branch additions during a 36 min imaging period. Red represents losses. Blue represents transient branches. F, Rates of branch addition, branch loss, and transient branches are significantly increased following LPS exposure (n = 21 untreated; n =18 LPS-treated). **p < 0.01; *p < 0.05; two-way repeated-measures ANOVA. Error bars indicate SEM. Scale bars, 25 µm. F, Fertilization.