Figure 6.

Effects of LPS on RGC arbor growth and elaboration are mediated in part through IL-1β. A, Branching dynamics are not affected by LPS treatment in the absence of IL-1β. The rate of branch additions and branch loss is not significantly different in LPS versus untreated larvae in IL-1β MO-injected animals (n = 8 untreated; n = 8 LPS); two-way repeated-measures ANOVA. B,E, LPS treatment does not significantly affect the growth of RGC arbors in IL-1β MO-injected larvae. Total arbor length (B) and branch number (C) are not significantly different from those of untreated control cells (n = 7 untreated, n = 7 LPS); two-way repeated-measures ANOVA. Single RGCs in embryos injected with IL-1β MO were imaged daily for 4 d following 2 h of mock treatment (IL-1β MO untreated, D) or LPS treatment (IL-1β MO + LPS, E) administered at 3 dpf. F,G, IL-1β MO validation. Embryos were injected with IL-1β−5′UTR-eGFP mRNA and control (F) or IL-1β MO (G). The number of larvae with green fluorescence and the total number of scored larvae are indicated at the bottom of each image. Error bars indicate SEM. Scale bars: D,E, 25 µm; F,G, 200 µm.