Figure 4.

Separation of gill cell populations by density gradient centrifugation, determination of the abundance of cell surface proteins and virus load in separated cell populations. (a) Gill- derived cells separated by Percoll gradient centrifugation. (b) Diagrammatic presentation of distinct populations of pavement cells (PC) goblet cells (GC) and mitochondria rich cells (MRC) in gill tissue. Cells isolated from infected gills harbored different mean levels of virus DNA. (c) Different levels of expression of mRNA encoding the P4a core protein of CEV. (d) Expression of the cellular markers mucin 2-like (muc2-like), occludin (ocldn), and rhesus glycoprotein type C (rhgc) in the different gill-derived cell populations. Letters (a, b) indicate significant differences at p ≤ 0.05 between different cell populations. Expression data for p4a are presented as box plots of 25% – 75% percentiles (± minimum and maximum values) with an indication of median as a vertical line. Mean host genes expression is presented as a bar (+SD). The diagram drown by authors based of MRC cells diagram by Degnan et al. ⁶⁶