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Staining of the tight junction protein-1 (ZO-1) (in red) in gills of CEV not infected (a) and CEV-infected (b) fish, showing severe clinical signs of KSD. Cell nuclei were counterstained with DAPI. CEV-infected gills show a severe occlusion of the intralamellar space, which blocks the contact of epithelial cells (ZO-1 positive) with water. Bar 50 µm
