Figure 11.

The mRNA expression levels of genes involved in immune responses in tissues of carp under CEV infection. (A, B, C) depicted are mRNA copy numbers of genes encoding surface protein CD4 of T helper cells (cd4), (D, E, F) surface protein CD8 b1 of cytotoxic T cells (cd8 b1), (G, H, I) T cell receptor a2 (tcr a2), (J, K, L) immunoglobulin M (igm), involved in adaptive immune responses in gills and kidney of koi and AS carp after exposure to koi infected with CEV genogroup IIa and kept in freshwater or water supplemented with 0.5% NaCl. Analysis was performed with one-way or two-way ANOVA with strain and time -points as factors. The ANOVA was followed by subsequent pairwise multiple comparisons using the Holm-Sidak method. Significant differences between the control and infected carp are marked with *at p ≤ 0.05, with ** at p ≤ 0.01, with *** at p ≤ 0.001. The data are presented as box plots of 25% – 75% percentiles (±minimum and maximum values) with an indication of median as a horizontal line from measurements of n = 6 specimen