Table 2.

Blood parameters in carp from the koi and AS strains during a CEV infection. Mild lack of oxygen, severe loss of ions and accumulation of ammonia could be noticed in KSD-affected koi, while not affected AS showed no significant changes in blood parameters. Significant differences between the control and infected specimens are marked in bold and with * at p ≤ 0.05, with ** at p ≤ 0.01, with *** at p ≤ 0.001. Letters (a, b) indicate significant differences between different carp strains at p ≤ 0.05. P – blood plasma, B – whole blood, FP – flame photometer, OPTI – Osmetech OPTI CCA Blood Gas Analyzer, PM – photometer, RF – refractometer, # indicates that sodium level was below levels which can be measured with the equipment

Factor	Instrument			Koi	AS	Koi	AS	Koi	AS	Koi	AS	AS
				Control	Control	CEV 3 dpe	CEV 3 dpe	CEV 6 dpe	CEV 6 dpe	CEV 9 dpe	CEV 9 dpe	CEV 13 dpe
pH	OPTI	B	Mean	7.35	7.35	7.37	7.39	7.34	7.38	7.44**	7.48	7.38
			SD	0.09	0.08	0.06	0.04	0.12	0.03	0.02	0.06	0.09
pCO2 (mmHg)	OPTI	B	Mean	22.50	21.67	28.83***a	24.00b	24.67	23.50	24.00a	19.83b	22.50
			SD	2.43	2.94	3.66	2.53	1.75	1.38	2.83	1.83	3.39
pO2 (mmHg)	OPTI	B	Mean	41.00	61.50	17.83**	31.17	15.17	35.17	16.00a	48.33b	41.83
			SD	17.56	21.93	9.17	19.18	6.82	20.07	6.83	20.09	17.05
BE (mmol L−1)	OPTI	B	Mean	−12.13	−12.38	−7.88*	−9.22	−11.12	−10.12	−6.98*	−7.53*	−10.80
			SD	3.22	2.25	2.78	2.25	4.91	1.53	2.08	3.05	2.35
tCO2 (mmol L−1)	OPTI	B	Mean	12.83	12.32	17.20*	14.97	14.48	14.27	16.80*	15.08	13.55
			SD	1.86	0.84	2.24	2.00	5.20	1.29	2.21	2.39	1.07
HCO3 (mmol L−1)	OPTI	B	Mean	12.12	11.65	16.32	14.25	13.68	13.57	13.05	14.47	12.88
			SD	1.90	0.90	2.20	1.96	5.15	1.23	7.81	2.35	1.11
Na+ (mmol L−1)	OPTI	B	Mean	139.00	135.67	125.33***a	137.67b	<100***a#	140.33b	115.75***a	139.17b	138.50
			SD	1.67	3.27	8.36	2.50		1.63	11.24	2.99	1.64
Na+ (mmol L−1)	FP	P	Mean	130.50	132.17	125.20	127.30	71.65***a	128.63b	112.40***a	126.68b	126.90
			SD	1.37	3.93	4.42	3.65	7.14	4.24	0.57	1.67	3.28
K+ (mmol L−1)	OPTI	B	Mean	2.73	2.57	3.50a	2.27b	4.32*a	2.65b	1.40	2.57	2.97
			SD	0.36	0.49	1.88	0.55	1.36	0.33	0.36	0.42	0.54
K+ (mmol L−1)	FP	P	Mean	2.86	3.14	2.11	2.05***	3.86*a	2.50b	1.23***a	2.85b	3.09
			SD	0.41	0.82	0.23	0.55	0.15	0.26	0.25	0.69	0.61
Ca++ (mmol L−1)	OPTI	B	Mean	1.34	1.35	1.30	1.32	1.03***a	1.45b	1.27a	1.38b	1.30
			SD	0.13	0.05	0.04	0.08	0.12	0.07	0.11	0.04	0.04
NH3 (µmol L−1)	PM	P	Mean	225.98	228.55	276.63a	172.12b	452.66***a	196.73b	131.23	154.38	270.30
			SD	100.92	72.57	24.31	20.52	95.66	63.90	20.63	50.44	111.69
Total protein (gL−1)	RF	P	Mean	24.88a	28.82b	25.43	27.18	30.76*	28.42	22.17a	28.74b	29.36
			SD	1.23	0.94	1.31	4.65	6.09	3.19	0.83	2.37	4.75
tHb (g L−1)	OPTI	B	Mean	87.00a	96.83b	97.20	100.17	86.17**a	94.67b	86.25a	95.67b	98.50
			SD	6.78	5.64	5.72	6.82	29.42	3.50	4.35	11.91	12.90
Hct© (%)	OPTI	B	Mean	26.17a	29.00b	29.20	30.33	31.25**	28.33	26.00	28.83	29.83
			SD	2.14	1.90	1.79	1.97	2.36	1.03	1.41	3.97	3.97