Table 3.
The effect of a CEV infection on mRNA expression of genes involved in gill transport and barrier functions. The onset of KSD is evident with a significantly lower expression of carbonic anhydrase 15a (ca15a) and the apical membrane epithelial calcium channel (ecac). This indicates a lower sodium and calcium ion import across the plasma membrane of gill cells in KSD-affected carp. Furthermore, CEV infection led to an increase in the transcription of aquaporin 3a (aqp3a), which could be related to enhanced water permeability of the gills. Shown are mean ± standard deviation of measurements from n = 6 specimen. Analysis was performed with one-way ANOVA, followed by subsequent pairwise multiple comparisons using the Holm-Sidak method. Significant differences between the control and infected carp are marked in bold and with * at p ≤ 0.05, with ** at p ≤ 0.01, with *** at p ≤ 0.001
| Gene | Protein name | Function | Time point | ||||
| Control | 3 dpe | 6 dpe | 9 dpe | ||||
| cdh1 | E-Cadherin | Tight Junction | |||||
| Mean | 7,66E+04 | 1,14E+05 | 1,08E+05 | 8,00E+04 | |||
| SD | 9,40E+03 | 6,48E+04 | 5,31E+03 | 4,28E+04 | |||
| ocldn a | Occludin | Tight Junction | |||||
| Mean | 1,04E+04 | 8,52E+03 | 3,94E+03 | 5,91E+03 | |||
| SD | 2,37E+03 | 5,71E+03 | 8,54E+02 | 2,82E+03 | |||
| kir1.1 | Na+,K+Cl− transporter | Ion transport | |||||
| Mean | 1,72E+03 | 1,13E+03 | 1,21E+03 | 1,69E+03 | |||
| SD | 7,72E+02 | 6,48E+02 | 4,46E+02 | 1,35E+03 | |||
| atp1.1.5 | Na+,K+ ATPase | Ion transport | |||||
| Mean | 5,96E+03 | 6,14E+03 | 8,96E+03 | 6,65E+03 | |||
| SD | 8,04E+02 | 4,02E+03 | 9,93E+02 | 2,20E+03 | |||
| rhcg | Rhesus C glycoprotein | Ammonia transport | |||||
| Mean | 7,31E+02 | 2,99E+03 | 5,98E+02 | 2,34E+03 | |||
| SD | 2,98E+02 | 3,81E+03 | 5,77E+02 | 3,63E+03 | |||
| ae1b1 | Na+HCO3− Co-transporter b1 | Ion transport | |||||
| Mean | 3,10E+03 | 2,37E+03 | 1,69E+03 | 3,98E+03 | |||
| SD | 9,49E+02 | 2,04E+03 | 8,24E+02 | 1,55E+03 | |||
| ae1b2 | Na+, HCO3− Co-transporter b2 | Ion transport | |||||
| Mean | 2,56E+01 | 1,27E+01 | 6,06E+01 | 9,40E+00 | |||
| SD | 1,01E+01 | 6,73E+00 | 9,59E+01 | 4,28E+00 | |||
| atph+ | H+ ATPase | Ion transport | |||||
| Mean | 7,49E+02 | 1,20E+03 | 7,72E+02 | 2,05E+03 | |||
| SD | 1,11E+02 | 9,97E+02 | 3,40E+02 | 2,90E+03 | |||
| ca15a | Carbonic anhydrase | Acid – base regulation | |||||
| Mean | 7,74E+03 | 3,68E+03 | 8,77E+02** | 1,62E+03* | |||
| SD | 4,87E+03 | 3,35E+03 | 7,02E+02 | 1,53E+03 | |||
| ncc | Sodium, Postassium, Chloride transporter | Ion transport | |||||
| Mean | 8,57E+02 | 1,81E+03 | 2,32E+03 | 2,12E+03 | |||
| SD | 2,61E+02 | 1,71E+03 | 2,41E+03 | 1,46E+03 | |||
| nhe3b | Sodium Hydrogen exchanger 3b | Ion transport | |||||
| Mean | 1,80E+00 | 2,80E+00 | 7,80E+00 | 1,59E+00 | |||
| SD | 3,32E+00 | 3,52E+00 | 1,02E+01 | 7,63E-01 | |||
| aqp3a | Aquaporin 3a | Water volume regulation | |||||
| Mean | 1,00E+04 | 3,39E+04 | 5,80E+04* | 1,73E+04 | |||
| SD | 2,57E+03 | 3,04E+04 | 7,21E+04 | 6,40E+03 | |||
| aqp3b | Aquaporin 3b | Water volume regulation | |||||
| Mean | 1,25E-01 | 9,92E-02 | 9,35E-01 | 7,99E-02 | |||
| SD | 1,67E-01 | 7,83E-02 | 1,84E+00 | 7,67E-02 | |||
| ecac | Epithelial Ca2+ channel | Ion transport | |||||
| Mean | 3,38E+02 | 4,14E+02 | 7,10E+01** | 4,50E+01*** | |||
| SD | 1,51E+02 | 3,16E+02 | 3,15E+01 | 2,85E+01 | |||